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emhc f1 652  (Developmental Studies Hybridoma Bank)


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    Developmental Studies Hybridoma Bank emhc f1 652
    Emhc F1 652, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/emhc+f1+652/anti-Myosin+heavy+chain/bio_rxiv__64898__2026__04__10__717808-196-48-50
    Average 95 stars, based on 18 article reviews
    emhc f1 652 - by Bioz Stars, 2026-09
    95/100 stars

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    Article Title: Alternative splicing misregulation secondary to skeletal muscle regeneration
    Article Snippet: The following antibodies were used for protein detection: CELF1 clone 3B1 (M. Swanson, 1:1,000) and CELF2 clone IH2 (M. Swanson, 1:500) followed by goat anti-mouse light chain (Jackson ImmunoResearch, 1:10,000); MBNL1 (C. Thornton, 1:1,000) followed by goat anti-rabbit (Calbiochem, 1:10,000); eMHC F1.652 (Developmental Studies Hybridoma Bank, 1:200) and GAPDH (Abcam, 1:5,000) followed by sheep anti-mouse (Jackson ImmunoResearch 1:10,000).

    Article Title: The Muscle Tissue Environment Limits Muscle Stem Cells in Aged Mice
    Article Snippet: Primary antibodies included mouse anti-Pax7 (Developmental Studies Hybridoma Bank, University of Iowa, USA) at 1:750, rabbit anti-myc-tag (CellSignaling) at 1:400, chicken anti-syndecan4 (Developmental Studies Hybridoma Bank, University of Iowa, USA) at 1:1000, mouse anti-phosphoFGFR1 (CellSignaling) at 1:100, phospho H3 (Millipore Sigma) at 1:500, myog F5D (abcam) at 1:1, emhc F1.652 (DSHB) at 1:5, anti-Synaptic vesicle glycoprotein 2A (Developmental Studies Hybridoma Bank, University of Iowa, USA), and anti-2H3 neurofilament (Developmental Studies Hybridoma Bank, University of Iowa, USA).

    Membrane:

    Article Title: CUGBP1 overexpression in mouse skeletal muscle reproduces features of myotonic dystrophy type 1
    Article Snippet: Protein concentration from the supernatant was determined with the BCA protein assay kit (Thermo Scientific). .. Protein samples (50 μg each) were separated on a 10% SDS–PAGE gel and transferred to Immobilon-P membrane (Millipore), after which the membranes were blocked and probed with one of the following antibodies: CUGBP1 clone 3B1 (M. Swanson, 1:1000) and CUGBP2 clone IH2 (M. Swanson, 1:500) followed by goat anti-mouse light chain (Jackson ImmunoResearch, 1:10 000); MBNL1 (C. Thornton, 1:1000) followed by goat anti-rabbit (Calbiochem, 1:10 000); eMHC F1.652 (Developmental Studies Hybridoma Bank, 1:200) and GAPDH (Abcam, 1:5000) followed by sheep anti-mouse (Jackson ImmunoResearch 1:10 000). .. Statistical analysis All statistical analysis was performed with GraphPad InStat software.

    Immunopeptidomics:

    Article Title: The exerkine apelin reverses age-associated sarcopenia.
    Article Snippet: 1Institut des Maladies Métaboliques et Cardiovasculaires, INSERM U1048, Université de Toulouse, Université Paul Sabatier, Toulouse, France.. 2Aging Department, Nestlé Institute of Health Sciences SA, Ecole Polytechnique Fédérale de Lausanne Innovation Park, Lausanne, Switzerland.. 3Institut de Pharmacologie et de Biologie Structurale–CNRS, Université de Toulouse, Université Paul Sabatier, Toulouse, France.



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    emhc f1 652  (Developmental Studies Hybridoma Bank)
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    Developmental Studies Hybridoma Bank emhc f1 652
    Emhc F1 652, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse anti embryonic myosin heavy chain emhc  (Developmental Studies Hybridoma Bank)
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    Developmental Studies Hybridoma Bank mouse anti embryonic myosin heavy chain emhc
    Ruxolitinib treatment decreased senescent macrophages and SASP in the skeletal muscle. A. GLB1/CD68 double immunofluorescent staining. GLB1 stained in red color, CD68 stained in green color. Double positive cells stained in yellow or orange color in merged images. B-D quantification of GLB1 + /CD68 + /200X field, GLB1 + /CD68 + cell percentage and GLB1 + /CD68 − cells/200X field. E. FUCA1/CD68 double immunofluorescent staining. FUCA1 stained in red color and CD68 stained in green color. Double positive cells showed in yellow or orange in merged images. F-H. Quantification of FUCA1 + /CD68 + , FUCA1 + /CD68 + cell percentage and FUCA + CD68 − cells. I. FUCA1/CD31 double positive cells to detect senescent endothelial cells. FUCA1 stained in red and CD31 stained in green. Double positive cells stained in orange or yellow. J-L. Quantification of FUCA1 + CD31 + cells/200X field, FUCA1 + CD31 + cells percentage, FUCA1 − CD31 + cells/200X field (non-senescent endothelial cells). M. CD68/P21 double staining. P21 stained red in the nuclei, CD68 stained green in the cytoplasm and membrane. Double positive cells stained in orange or yellow in merged images. N-P. Quantification of CD68 + /P21 + cells/200X field, CD68 + /P21 + cell percentage and CD68 − P21 + cells/200X field (non-macrophage senescent cells). Q. <t>eMHC/GLB1</t> double immunofluorescence staining. eMHC + cells stained bright green, GLB1 + cells stained red. eMHC + cells were almost undetectable in the control group and very few cells were detected in the muscle tissue of the ruxolitinib-treated group. eMHC did not colocalize with GLB1. R-S. PAX7/GLB1 double immunofluorescence staining and quantification. PAX7 did not colocalize with GLB1. Very few PAX + /GLB1 − cells were detected in the control group, but relatively more were detected in the ruxolitinib-treated group. T. H&E staining showed the most severe damage area of each group in the gastrocnemius muscle. U-X. Q-PCR analysis of GLB1, MIF, TRAP and BMP4 mRNA expression in the thigh muscle. Y. Q-PCR analysis of M1 and M2 macrophage markers as well as PAX7, MYOG and MYOD mRNA expression. N = 8 for each group, including males and females. Exact P values are indicated between group bars. Scale bars= 100 μm for 200X and 200 μm for 400X (PAX7).
    Mouse Anti Embryonic Myosin Heavy Chain Emhc, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    emhc  (Developmental Studies Hybridoma Bank)
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    Developmental Studies Hybridoma Bank emhc
    Ruxolitinib treatment decreased senescent macrophages and SASP in the skeletal muscle. A. GLB1/CD68 double immunofluorescent staining. GLB1 stained in red color, CD68 stained in green color. Double positive cells stained in yellow or orange color in merged images. B-D quantification of GLB1 + /CD68 + /200X field, GLB1 + /CD68 + cell percentage and GLB1 + /CD68 − cells/200X field. E. FUCA1/CD68 double immunofluorescent staining. FUCA1 stained in red color and CD68 stained in green color. Double positive cells showed in yellow or orange in merged images. F-H. Quantification of FUCA1 + /CD68 + , FUCA1 + /CD68 + cell percentage and FUCA + CD68 − cells. I. FUCA1/CD31 double positive cells to detect senescent endothelial cells. FUCA1 stained in red and CD31 stained in green. Double positive cells stained in orange or yellow. J-L. Quantification of FUCA1 + CD31 + cells/200X field, FUCA1 + CD31 + cells percentage, FUCA1 − CD31 + cells/200X field (non-senescent endothelial cells). M. CD68/P21 double staining. P21 stained red in the nuclei, CD68 stained green in the cytoplasm and membrane. Double positive cells stained in orange or yellow in merged images. N-P. Quantification of CD68 + /P21 + cells/200X field, CD68 + /P21 + cell percentage and CD68 − P21 + cells/200X field (non-macrophage senescent cells). Q. <t>eMHC/GLB1</t> double immunofluorescence staining. eMHC + cells stained bright green, GLB1 + cells stained red. eMHC + cells were almost undetectable in the control group and very few cells were detected in the muscle tissue of the ruxolitinib-treated group. eMHC did not colocalize with GLB1. R-S. PAX7/GLB1 double immunofluorescence staining and quantification. PAX7 did not colocalize with GLB1. Very few PAX + /GLB1 − cells were detected in the control group, but relatively more were detected in the ruxolitinib-treated group. T. H&E staining showed the most severe damage area of each group in the gastrocnemius muscle. U-X. Q-PCR analysis of GLB1, MIF, TRAP and BMP4 mRNA expression in the thigh muscle. Y. Q-PCR analysis of M1 and M2 macrophage markers as well as PAX7, MYOG and MYOD mRNA expression. N = 8 for each group, including males and females. Exact P values are indicated between group bars. Scale bars= 100 μm for 200X and 200 μm for 400X (PAX7).
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    mouse anti emhc  (Developmental Studies Hybridoma Bank)
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    Ruxolitinib treatment decreased senescent macrophages and SASP in the skeletal muscle. A. GLB1/CD68 double immunofluorescent staining. GLB1 stained in red color, CD68 stained in green color. Double positive cells stained in yellow or orange color in merged images. B-D quantification of GLB1 + /CD68 + /200X field, GLB1 + /CD68 + cell percentage and GLB1 + /CD68 − cells/200X field. E. FUCA1/CD68 double immunofluorescent staining. FUCA1 stained in red color and CD68 stained in green color. Double positive cells showed in yellow or orange in merged images. F-H. Quantification of FUCA1 + /CD68 + , FUCA1 + /CD68 + cell percentage and FUCA + CD68 − cells. I. FUCA1/CD31 double positive cells to detect senescent endothelial cells. FUCA1 stained in red and CD31 stained in green. Double positive cells stained in orange or yellow. J-L. Quantification of FUCA1 + CD31 + cells/200X field, FUCA1 + CD31 + cells percentage, FUCA1 − CD31 + cells/200X field (non-senescent endothelial cells). M. CD68/P21 double staining. P21 stained red in the nuclei, CD68 stained green in the cytoplasm and membrane. Double positive cells stained in orange or yellow in merged images. N-P. Quantification of CD68 + /P21 + cells/200X field, CD68 + /P21 + cell percentage and CD68 − P21 + cells/200X field (non-macrophage senescent cells). Q. <t>eMHC/GLB1</t> double immunofluorescence staining. eMHC + cells stained bright green, GLB1 + cells stained red. eMHC + cells were almost undetectable in the control group and very few cells were detected in the muscle tissue of the ruxolitinib-treated group. eMHC did not colocalize with GLB1. R-S. PAX7/GLB1 double immunofluorescence staining and quantification. PAX7 did not colocalize with GLB1. Very few PAX + /GLB1 − cells were detected in the control group, but relatively more were detected in the ruxolitinib-treated group. T. H&E staining showed the most severe damage area of each group in the gastrocnemius muscle. U-X. Q-PCR analysis of GLB1, MIF, TRAP and BMP4 mRNA expression in the thigh muscle. Y. Q-PCR analysis of M1 and M2 macrophage markers as well as PAX7, MYOG and MYOD mRNA expression. N = 8 for each group, including males and females. Exact P values are indicated between group bars. Scale bars= 100 μm for 200X and 200 μm for 400X (PAX7).
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    embryonic myosin heavy chain emhc  (Developmental Studies Hybridoma Bank)
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    Ruxolitinib treatment decreased senescent macrophages and SASP in the skeletal muscle. A. GLB1/CD68 double immunofluorescent staining. GLB1 stained in red color, CD68 stained in green color. Double positive cells stained in yellow or orange color in merged images. B-D quantification of GLB1 + /CD68 + /200X field, GLB1 + /CD68 + cell percentage and GLB1 + /CD68 − cells/200X field. E. FUCA1/CD68 double immunofluorescent staining. FUCA1 stained in red color and CD68 stained in green color. Double positive cells showed in yellow or orange in merged images. F-H. Quantification of FUCA1 + /CD68 + , FUCA1 + /CD68 + cell percentage and FUCA + CD68 − cells. I. FUCA1/CD31 double positive cells to detect senescent endothelial cells. FUCA1 stained in red and CD31 stained in green. Double positive cells stained in orange or yellow. J-L. Quantification of FUCA1 + CD31 + cells/200X field, FUCA1 + CD31 + cells percentage, FUCA1 − CD31 + cells/200X field (non-senescent endothelial cells). M. CD68/P21 double staining. P21 stained red in the nuclei, CD68 stained green in the cytoplasm and membrane. Double positive cells stained in orange or yellow in merged images. N-P. Quantification of CD68 + /P21 + cells/200X field, CD68 + /P21 + cell percentage and CD68 − P21 + cells/200X field (non-macrophage senescent cells). Q. <t>eMHC/GLB1</t> double immunofluorescence staining. eMHC + cells stained bright green, GLB1 + cells stained red. eMHC + cells were almost undetectable in the control group and very few cells were detected in the muscle tissue of the ruxolitinib-treated group. eMHC did not colocalize with GLB1. R-S. PAX7/GLB1 double immunofluorescence staining and quantification. PAX7 did not colocalize with GLB1. Very few PAX + /GLB1 − cells were detected in the control group, but relatively more were detected in the ruxolitinib-treated group. T. H&E staining showed the most severe damage area of each group in the gastrocnemius muscle. U-X. Q-PCR analysis of GLB1, MIF, TRAP and BMP4 mRNA expression in the thigh muscle. Y. Q-PCR analysis of M1 and M2 macrophage markers as well as PAX7, MYOG and MYOD mRNA expression. N = 8 for each group, including males and females. Exact P values are indicated between group bars. Scale bars= 100 μm for 200X and 200 μm for 400X (PAX7).
    Embryonic Myosin Heavy Chain Emhc, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    emhc mouse dhsb f1 652  (Developmental Studies Hybridoma Bank)
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    Developmental Studies Hybridoma Bank emhc mouse dhsb f1 652
    Ruxolitinib treatment decreased senescent macrophages and SASP in the skeletal muscle. A. GLB1/CD68 double immunofluorescent staining. GLB1 stained in red color, CD68 stained in green color. Double positive cells stained in yellow or orange color in merged images. B-D quantification of GLB1 + /CD68 + /200X field, GLB1 + /CD68 + cell percentage and GLB1 + /CD68 − cells/200X field. E. FUCA1/CD68 double immunofluorescent staining. FUCA1 stained in red color and CD68 stained in green color. Double positive cells showed in yellow or orange in merged images. F-H. Quantification of FUCA1 + /CD68 + , FUCA1 + /CD68 + cell percentage and FUCA + CD68 − cells. I. FUCA1/CD31 double positive cells to detect senescent endothelial cells. FUCA1 stained in red and CD31 stained in green. Double positive cells stained in orange or yellow. J-L. Quantification of FUCA1 + CD31 + cells/200X field, FUCA1 + CD31 + cells percentage, FUCA1 − CD31 + cells/200X field (non-senescent endothelial cells). M. CD68/P21 double staining. P21 stained red in the nuclei, CD68 stained green in the cytoplasm and membrane. Double positive cells stained in orange or yellow in merged images. N-P. Quantification of CD68 + /P21 + cells/200X field, CD68 + /P21 + cell percentage and CD68 − P21 + cells/200X field (non-macrophage senescent cells). Q. <t>eMHC/GLB1</t> double immunofluorescence staining. eMHC + cells stained bright green, GLB1 + cells stained red. eMHC + cells were almost undetectable in the control group and very few cells were detected in the muscle tissue of the ruxolitinib-treated group. eMHC did not colocalize with GLB1. R-S. PAX7/GLB1 double immunofluorescence staining and quantification. PAX7 did not colocalize with GLB1. Very few PAX + /GLB1 − cells were detected in the control group, but relatively more were detected in the ruxolitinib-treated group. T. H&E staining showed the most severe damage area of each group in the gastrocnemius muscle. U-X. Q-PCR analysis of GLB1, MIF, TRAP and BMP4 mRNA expression in the thigh muscle. Y. Q-PCR analysis of M1 and M2 macrophage markers as well as PAX7, MYOG and MYOD mRNA expression. N = 8 for each group, including males and females. Exact P values are indicated between group bars. Scale bars= 100 μm for 200X and 200 μm for 400X (PAX7).
    Emhc Mouse Dhsb F1 652, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 1 article reviews
    emhc mouse dhsb f1 652 - by Bioz Stars, 2026-09
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    anti myh3 emhc  (Developmental Studies Hybridoma Bank)
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    Developmental Studies Hybridoma Bank anti myh3 emhc
    Ruxolitinib treatment decreased senescent macrophages and SASP in the skeletal muscle. A. GLB1/CD68 double immunofluorescent staining. GLB1 stained in red color, CD68 stained in green color. Double positive cells stained in yellow or orange color in merged images. B-D quantification of GLB1 + /CD68 + /200X field, GLB1 + /CD68 + cell percentage and GLB1 + /CD68 − cells/200X field. E. FUCA1/CD68 double immunofluorescent staining. FUCA1 stained in red color and CD68 stained in green color. Double positive cells showed in yellow or orange in merged images. F-H. Quantification of FUCA1 + /CD68 + , FUCA1 + /CD68 + cell percentage and FUCA + CD68 − cells. I. FUCA1/CD31 double positive cells to detect senescent endothelial cells. FUCA1 stained in red and CD31 stained in green. Double positive cells stained in orange or yellow. J-L. Quantification of FUCA1 + CD31 + cells/200X field, FUCA1 + CD31 + cells percentage, FUCA1 − CD31 + cells/200X field (non-senescent endothelial cells). M. CD68/P21 double staining. P21 stained red in the nuclei, CD68 stained green in the cytoplasm and membrane. Double positive cells stained in orange or yellow in merged images. N-P. Quantification of CD68 + /P21 + cells/200X field, CD68 + /P21 + cell percentage and CD68 − P21 + cells/200X field (non-macrophage senescent cells). Q. <t>eMHC/GLB1</t> double immunofluorescence staining. eMHC + cells stained bright green, GLB1 + cells stained red. eMHC + cells were almost undetectable in the control group and very few cells were detected in the muscle tissue of the ruxolitinib-treated group. eMHC did not colocalize with GLB1. R-S. PAX7/GLB1 double immunofluorescence staining and quantification. PAX7 did not colocalize with GLB1. Very few PAX + /GLB1 − cells were detected in the control group, but relatively more were detected in the ruxolitinib-treated group. T. H&E staining showed the most severe damage area of each group in the gastrocnemius muscle. U-X. Q-PCR analysis of GLB1, MIF, TRAP and BMP4 mRNA expression in the thigh muscle. Y. Q-PCR analysis of M1 and M2 macrophage markers as well as PAX7, MYOG and MYOD mRNA expression. N = 8 for each group, including males and females. Exact P values are indicated between group bars. Scale bars= 100 μm for 200X and 200 μm for 400X (PAX7).
    Anti Myh3 Emhc, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/emhc+f1+652/anti-Myosin+heavy+chain/pmc11734708-75-58-64
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    Ruxolitinib treatment decreased senescent macrophages and SASP in the skeletal muscle. A. GLB1/CD68 double immunofluorescent staining. GLB1 stained in red color, CD68 stained in green color. Double positive cells stained in yellow or orange color in merged images. B-D quantification of GLB1 + /CD68 + /200X field, GLB1 + /CD68 + cell percentage and GLB1 + /CD68 − cells/200X field. E. FUCA1/CD68 double immunofluorescent staining. FUCA1 stained in red color and CD68 stained in green color. Double positive cells showed in yellow or orange in merged images. F-H. Quantification of FUCA1 + /CD68 + , FUCA1 + /CD68 + cell percentage and FUCA + CD68 − cells. I. FUCA1/CD31 double positive cells to detect senescent endothelial cells. FUCA1 stained in red and CD31 stained in green. Double positive cells stained in orange or yellow. J-L. Quantification of FUCA1 + CD31 + cells/200X field, FUCA1 + CD31 + cells percentage, FUCA1 − CD31 + cells/200X field (non-senescent endothelial cells). M. CD68/P21 double staining. P21 stained red in the nuclei, CD68 stained green in the cytoplasm and membrane. Double positive cells stained in orange or yellow in merged images. N-P. Quantification of CD68 + /P21 + cells/200X field, CD68 + /P21 + cell percentage and CD68 − P21 + cells/200X field (non-macrophage senescent cells). Q. eMHC/GLB1 double immunofluorescence staining. eMHC + cells stained bright green, GLB1 + cells stained red. eMHC + cells were almost undetectable in the control group and very few cells were detected in the muscle tissue of the ruxolitinib-treated group. eMHC did not colocalize with GLB1. R-S. PAX7/GLB1 double immunofluorescence staining and quantification. PAX7 did not colocalize with GLB1. Very few PAX + /GLB1 − cells were detected in the control group, but relatively more were detected in the ruxolitinib-treated group. T. H&E staining showed the most severe damage area of each group in the gastrocnemius muscle. U-X. Q-PCR analysis of GLB1, MIF, TRAP and BMP4 mRNA expression in the thigh muscle. Y. Q-PCR analysis of M1 and M2 macrophage markers as well as PAX7, MYOG and MYOD mRNA expression. N = 8 for each group, including males and females. Exact P values are indicated between group bars. Scale bars= 100 μm for 200X and 200 μm for 400X (PAX7).

    Journal: Pharmacological research

    Article Title: Targeting cellular senescence in dystrophin −/− /utrophin −/− double knockout mice improves musculoskeletal health and increases lifespan ☆

    doi: 10.1016/j.phrs.2025.108016

    Figure Lengend Snippet: Ruxolitinib treatment decreased senescent macrophages and SASP in the skeletal muscle. A. GLB1/CD68 double immunofluorescent staining. GLB1 stained in red color, CD68 stained in green color. Double positive cells stained in yellow or orange color in merged images. B-D quantification of GLB1 + /CD68 + /200X field, GLB1 + /CD68 + cell percentage and GLB1 + /CD68 − cells/200X field. E. FUCA1/CD68 double immunofluorescent staining. FUCA1 stained in red color and CD68 stained in green color. Double positive cells showed in yellow or orange in merged images. F-H. Quantification of FUCA1 + /CD68 + , FUCA1 + /CD68 + cell percentage and FUCA + CD68 − cells. I. FUCA1/CD31 double positive cells to detect senescent endothelial cells. FUCA1 stained in red and CD31 stained in green. Double positive cells stained in orange or yellow. J-L. Quantification of FUCA1 + CD31 + cells/200X field, FUCA1 + CD31 + cells percentage, FUCA1 − CD31 + cells/200X field (non-senescent endothelial cells). M. CD68/P21 double staining. P21 stained red in the nuclei, CD68 stained green in the cytoplasm and membrane. Double positive cells stained in orange or yellow in merged images. N-P. Quantification of CD68 + /P21 + cells/200X field, CD68 + /P21 + cell percentage and CD68 − P21 + cells/200X field (non-macrophage senescent cells). Q. eMHC/GLB1 double immunofluorescence staining. eMHC + cells stained bright green, GLB1 + cells stained red. eMHC + cells were almost undetectable in the control group and very few cells were detected in the muscle tissue of the ruxolitinib-treated group. eMHC did not colocalize with GLB1. R-S. PAX7/GLB1 double immunofluorescence staining and quantification. PAX7 did not colocalize with GLB1. Very few PAX + /GLB1 − cells were detected in the control group, but relatively more were detected in the ruxolitinib-treated group. T. H&E staining showed the most severe damage area of each group in the gastrocnemius muscle. U-X. Q-PCR analysis of GLB1, MIF, TRAP and BMP4 mRNA expression in the thigh muscle. Y. Q-PCR analysis of M1 and M2 macrophage markers as well as PAX7, MYOG and MYOD mRNA expression. N = 8 for each group, including males and females. Exact P values are indicated between group bars. Scale bars= 100 μm for 200X and 200 μm for 400X (PAX7).

    Article Snippet: Primary antibodies and their dilutions were as listed below: rat anti-CD68 (ab53444, Abcam, 1:200 dilution), rabbit anti-β-galactosidase (GLB1) Cat#:15518–1-AP, Proteintech, 1:200 dilution), rabbit anti-alpha-L-fucosidase (FUCA1) (Catalog# PA5–115256, ThermoFisher Scientific, 1:200 dilution), rabbit anti-P21 (ab188224, Abcam, 1:400 dilution), rat anti-CD31(BD553370, BD Biosciences, 1:300 dilution), mouse anti-embryonic myosin heavy chain (eMHC) (F1.652, DSHB, 1:50 dilution) and paired box transcription factor 7 (PAX7) (DSHB, 1:100 dilution).

    Techniques: Staining, Double Staining, Membrane, Double Immunofluorescence Staining, Control, Expressing

    Synergistic beneficial effect of ruxolitinib and deflazacort on skeletal muscle tissues. A-B. H&E staining at 40X and 100X magnification. Large amounts of inflammatory cells are present in the gastrocnemius muscle tissues in the vehicle-treated group. Deflazacort decreased inflammatory cells, but had no effect on heterotopic bone formation (dark red patch-like staining), while the D+R group obviously decreased inflammatory cells and HO formation and improved muscle pathology. C. GLB1/CD68 double immunofluorescence staining of gastrocnemius muscle. GLB1 stained red, CD68 stained green. Double positive cells stained orange or yellow in merged images. Insets highlight double positive cells. D-G. Quantification of CD68 + /GLB1 + /200X field, CD68 + /GLB1 + /total CD68 + percentage, CD68 + GLB1 − cells/200X field, Total CD68 + cells. H. Double immunofluorescence staining of eMHC and GLB1. eMHC stained green and GLB1 stained red. eMHC does not colocalize with GLB1. I. PAX7/GLB1 double immunofluorescence staining. PAX7 stained green in the nuclei and GLB1 stained red in cytoplasm. J. Quantification of PAX7 + /GLB1 − positive cells. K-L. Q-PCR analysis of M1 macrophage marker iNOS and CD86. M. Q-PCR analysis of M2 macrophage marker CD206. N-P. Q-PCR analysis of myogenic transcription factors PAX7, MYOG and MYOD. Q. Q-PCR analysis of MIF. Scale bars = 250 μm for 40X, 100 μm for 100X and 200X magnification and 50 μm for 400X magnification. N = 8–11 for different groups. Exact P values are indicated between group bars.

    Journal: Pharmacological research

    Article Title: Targeting cellular senescence in dystrophin −/− /utrophin −/− double knockout mice improves musculoskeletal health and increases lifespan ☆

    doi: 10.1016/j.phrs.2025.108016

    Figure Lengend Snippet: Synergistic beneficial effect of ruxolitinib and deflazacort on skeletal muscle tissues. A-B. H&E staining at 40X and 100X magnification. Large amounts of inflammatory cells are present in the gastrocnemius muscle tissues in the vehicle-treated group. Deflazacort decreased inflammatory cells, but had no effect on heterotopic bone formation (dark red patch-like staining), while the D+R group obviously decreased inflammatory cells and HO formation and improved muscle pathology. C. GLB1/CD68 double immunofluorescence staining of gastrocnemius muscle. GLB1 stained red, CD68 stained green. Double positive cells stained orange or yellow in merged images. Insets highlight double positive cells. D-G. Quantification of CD68 + /GLB1 + /200X field, CD68 + /GLB1 + /total CD68 + percentage, CD68 + GLB1 − cells/200X field, Total CD68 + cells. H. Double immunofluorescence staining of eMHC and GLB1. eMHC stained green and GLB1 stained red. eMHC does not colocalize with GLB1. I. PAX7/GLB1 double immunofluorescence staining. PAX7 stained green in the nuclei and GLB1 stained red in cytoplasm. J. Quantification of PAX7 + /GLB1 − positive cells. K-L. Q-PCR analysis of M1 macrophage marker iNOS and CD86. M. Q-PCR analysis of M2 macrophage marker CD206. N-P. Q-PCR analysis of myogenic transcription factors PAX7, MYOG and MYOD. Q. Q-PCR analysis of MIF. Scale bars = 250 μm for 40X, 100 μm for 100X and 200X magnification and 50 μm for 400X magnification. N = 8–11 for different groups. Exact P values are indicated between group bars.

    Article Snippet: Primary antibodies and their dilutions were as listed below: rat anti-CD68 (ab53444, Abcam, 1:200 dilution), rabbit anti-β-galactosidase (GLB1) Cat#:15518–1-AP, Proteintech, 1:200 dilution), rabbit anti-alpha-L-fucosidase (FUCA1) (Catalog# PA5–115256, ThermoFisher Scientific, 1:200 dilution), rabbit anti-P21 (ab188224, Abcam, 1:400 dilution), rat anti-CD31(BD553370, BD Biosciences, 1:300 dilution), mouse anti-embryonic myosin heavy chain (eMHC) (F1.652, DSHB, 1:50 dilution) and paired box transcription factor 7 (PAX7) (DSHB, 1:100 dilution).

    Techniques: Staining, Double Immunofluorescence Staining, Marker